What are the Different Types of Fluorescence Microscopy?
Several types of fluorescence microscopy techniques are commonly used in cell biology:
- Widefield Fluorescence Microscopy: Provides a broad view of the specimen by illuminating the entire field of view simultaneously. - Confocal Microscopy: Uses a pinhole to exclude out-of-focus light, providing higher resolution and contrast. - Two-Photon Microscopy: Utilizes two photons of longer wavelength light for excitation, reducing photodamage and allowing deeper tissue imaging. - Total Internal Reflection Fluorescence (TIRF) Microscopy: Excites fluorophores only near the surface of the specimen, ideal for studying membrane-associated processes. - Super-Resolution Microscopy: Techniques like STORM and PALM break the diffraction limit, providing extremely high resolution.
