Fluorescence microscopy involves exciting a fluorophore with a specific wavelength of light. The fluorophore then emits light at a longer wavelength, which can be detected and visualized. The process typically involves:
1. Excitation Light: A light source, such as a laser or a high-intensity lamp, emits light at the excitation wavelength. 2. Fluorophore Excitation: The excitation light is directed onto the specimen, causing the fluorophore to emit light. 3. Emission Detection: The emitted light is collected by a detector, such as a camera or photomultiplier tube, after passing through an emission filter that blocks the excitation light.
