While molecular cloning is a powerful technique, it comes with several challenges:
High Fidelity: Ensuring the accuracy of the inserted DNA sequence is critical, as errors can affect the function of the cloned gene. Transformation Efficiency: Not all host cells will successfully take up the recombinant DNA, making selection an important step. Expression Issues: Cloned genes may not always express correctly or efficiently in the host cells, especially in eukaryotic cells.
