The process of culturing cells involves several steps:
Isolation: Cells are isolated from tissues using enzymatic or mechanical methods. Seeding: Isolated cells are placed into culture media that provides essential nutrients, growth factors, and hormones. Incubation: Cultures are incubated at optimal conditions (temperature, CO2 levels) to promote cell growth. Sub-culturing: Cells are periodically transferred to new media to prevent overcrowding and maintain their health.