The process of molecular cloning involves several critical steps:
Isolation of DNA: The DNA fragment of interest is isolated from the biological source. Insertion into a Vector: The isolated DNA fragment is inserted into a cloning vector, which is a DNA molecule capable of replication in a host cell. Transformation: The recombinant DNA is introduced into a host cell through a process called transformation. Selection: Host cells that have taken up the recombinant DNA are selected using markers such as antibiotic resistance. Verification: The presence and correctness of the inserted DNA are verified, often through DNA sequencing.
