Glycolysis is tightly regulated to meet the energy demands of the cell. Key regulatory points include:
Hexokinase: Inhibited by its product, glucose-6-phosphate. Phosphofructokinase-1 (PFK-1): The main regulatory enzyme, allosterically inhibited by ATP and activated by AMP and fructose-2,6-bisphosphate. Pyruvate Kinase: Inhibited by ATP and alanine, activated by fructose-1,6-bisphosphate.
