Several advanced techniques are employed in proteomics, including:
Mass Spectrometry (MS): A crucial tool for identifying and quantifying proteins. MS provides detailed information about the molecular weight and structure of proteins. Two-Dimensional Gel Electrophoresis (2-DE): Separates proteins based on their isoelectric point and molecular weight, allowing for the analysis of protein mixtures. Liquid Chromatography (LC): Often coupled with mass spectrometry (LC-MS), this technique separates proteins or peptides in a mixture based on their chemical properties. Western Blotting: Used for the detection and quantification of specific proteins within a mixture.
