Despite its advantages, super resolution microscopy also faces several challenges:
Photobleaching: Prolonged exposure to high-intensity light can cause fluorophores to lose their ability to fluoresce. Sample Preparation: Preparing samples for super resolution microscopy can be complex and time-consuming. Imaging Speed: Some techniques, like PALM/STORM, require extended periods to acquire enough data for high-resolution images. Complexity of Data Analysis: The data generated can be immense and requires sophisticated algorithms for accurate interpretation.
