super resolution microscopy

What Are the Main Types of Super Resolution Microscopy?

Several techniques exist under the umbrella of super resolution microscopy, each with its own unique advantages and limitations:

STED (Stimulated Emission Depletion): This method uses a second laser to deplete fluorescence around a focal point, leading to improved resolution.
PALM (Photo-Activated Localization Microscopy) and STORM (Stochastic Optical Reconstruction Microscopy): Both techniques rely on the precise localization of individual fluorescent molecules to build a high-resolution image.
SIM (Structured Illumination Microscopy): This technique uses patterned illumination to enhance resolution.

Frequently asked queries:

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