The main components of a confocal microscope include:
1. Laser Light Source: Provides the illumination required for fluorescence excitation. 2. Scanning System: Moves the laser beam across the specimen. 3. Objective Lens: Focuses the laser beam onto the specimen and collects emitted light. 4. Dichroic Mirrors: Directs the excitation light to the specimen and the emitted light to the detector. 5. Pinhole Aperture: Blocks out-of-focus light. 6. Detector: Captures the in-focus fluorescent light to generate the image.
